Journal: Journal of Cancer Research and Clinical Oncology
Article Title: TREM1 + tumor-associated macrophages secrete CCL7 to promote hepatocellular carcinoma metastasis
doi: 10.1007/s00432-024-05831-1
Figure Lengend Snippet: CCL7 derived from TREM1 + tumor-associated macrophages promote hepatocellular carcinoma metastasis. A , B . The differential expression cytokine of TREM1 manipulation in THP1-derived TAMs was detected using Human Cytokine Array. C . The concentration of CCL7 in condition medium of M2 subtype TAMs, M0 subtype TAMs, and hepatocellular carcinoma cells was detected. D . TREM1 knockdown inhibited the CCL7 expression in THP1-derived TAMs. E . The condition medium of THP1/rTREM1-derived TAMs can promote the EMT of hepatocellular carcinoma cells, while CCL7-NA abrogated that. All values are presented as the mean ± SD. n = 3, * p < 0.05, ** p < 0.01
Article Snippet: Human Cytokine Array C5 kit (AAH-CYT-5-2, RayBiotech, Inc., Guangzhou) was carried out according to the manufacturer’s instructions.
Techniques: Derivative Assay, Expressing, Concentration Assay, Knockdown